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Determination of Significance：
Sucrose is not only an important photosynthetic product, but also a major transport material in plants. Moreover, it is one of the storage forms of carbohydrates. Sucrose phosphate synthase (SPS) takes fructose-6-phosphate as the receptor, the sucrose produced by the reaction forms sucrose phosphate under the action of sucrose phosphatase. Sucrose phosphate synthase-sucrose phosphatase system is generally regarded as the main route of sucrose synthesis.
Measurement Principle：
Sucrose phosphate synthase catalyzes fructose-6-phosphate to form sucrose phosphoric acid. The reaction between sucrose and resorcinol can present color change, which has a characteristic absorption peak at 480nm and the enzyme activity is proportional to the depth of color.
Self Provided：
Spectrophotometer, water-bath, table centrifuge, adjustable pipette, 1 mL glass cuvette, mortar/homogenizer, ice and distilled water.
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