Determination of Significance:
Triglyceride(TG) is a fat molecule formed by long-chain fatty acids and glycerol, which is not only the main component of cell membrane, but also an important respiratory substrate.
Measurement Principle:
The TG is extracted with isopropyl alcohol, then hydrolysis to glycerol and fatty acids after saponification of TG by KOH. Glycerol is oxidized by periodic acid to form formaldehyde. Condensation of formaldehyde and acetylacetone to form yellow components in presence of chloride ions. The yellow component has a characteristic absorption at 420 nm and proportional to the TG content.
Self Provided:
Spectrophotometer, water bath, adjustable pipette, 1 mL glass cuvette, n-heptane, isopropyl alcohol, distilled water, 100 mL empty glass bottle.
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