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NADH Oxidase(NOX) Activity Assay Kit

Storage:Store at -20℃,6 months

NADH Oxidase(NOX) Activity Assay Kit
Cat.No:
BC0630
Brand:
Solarbio

Determination of Significance:

NADH oxidase (NOX) (EC 1.6.99.3) exists widely in animals, plants, microorganisms and cultured cells, which can direct the oxidation of NADH to NAD under oxygen. This enzyme is not only involved in the regeneration of NAD, but also closely related to immune response.

Measurement Principle:

NOX can oxidize NADH to NAD, oxidation of NADH is coupled to the reduction phase of 2,6-dichlorophenol indigo (DCPIP) and the blue DCPIP is restored to colorless DCPIP. The NOX activity can be quantified by measuring the decrease in the color development at 600 nm.

Self Provided:

Spectrophotometer, water bath, desk centrifuge, adjustable pipette, 1 mL glass cuvette, mortar/homogenizer, ice and distilled water.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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