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Determination of Significance：
L-Lactate dehydrogenase (L-LDH or LD) is the terminal enzyme of the glycolysis pathway which is found in nearly all living cells (animals, plants, and prokaryotes). L-LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back.
Measurement Principle：
NAD+ and lactic acid is oxidized to pyruvic acid by the catalysis of L-LDH. Pyruvate further reacted with 2,4-dinitrophenylhydrazide to form pyruvate dinitrobenzone, which show brown red color in alkaline solution and the color depth is proportional to the concentration of pyruvate.
Self Provided：
Spectrophotometer, thermostat water bath, desk centrifuge, adjustable pipette, 1 mL cuvette, mortar/homogenizer, ice, distilled water.
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