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Determination of Significance：
Hexokinase (HK, EC 2.7.1.1) is widely distributed in animals, plants, microorganisms and cultured cells. It is the first key enzyme in the process of glucose decomposition, catalyzing the conversion of glucose into glucose 6-phosphate, which is the intersection of glycolysis and pentose phosphate pathways.
Measurement Principle：
HK catalyzes the synthesis of glucose to 6-phosphate glucose, and 6-phosphate glucose dehydrogenase further catalyzes the dehydrogenation of 6-phosphate glucose to NADPH, which has a characteristic absorption peak at 340 nm.
Self Provided：
Spectrophotometer, water bath, centrifuge, adjustable pipette, 1 mL quartz cuvette, mortar/ homogenizer, ice and distilled water.
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