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Determination of Significance：
Soil protease take part in the transform of amino acid, protein and other organic compounds contain protein nitrogen in soil. The products are one of the nitrogen sources in higher plants. Soil alkaline protease catalyzes hydrolysis of protein in alkaline condition which is related to soil organic content, nitrogen and other soil properties.
Measurement Principle：
In alkaline condition, soil alkaline protease can hydrolyze tyrosine to produce tyrosine. Tyrosine reduce phosphomolybdate compound to tungsten blue in alkaline condition, which has a absorption peak in 680 nm.
Self Provided：
Spectrophotometer, water-bath, adjustable pipette, 1 mL glass cuvette, methylbenzene, distilled water and 50 meshes sieve (or smaller).
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