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Determination of Significance：
Glutamine synthetase(GS) exists mainly in plants, is one of the key enzymes of ammonia assimilation in organism, which can catalytic synthesis of glutamine by ammonium ion and glutamic acid. The synthesis of glutamine not only prevents excessive ammonium ions from being toxic to organisms, but glutamine is also the main storage and transport form of ammonia.
Measurement Principle：
GS catalyzes the synthesis of glutamine from ammonium and glutamic acid in the presence of ATP and Mg2+. Glutamine is further converted to gamma-glutamyl hydroxamic acid, which can form a red complex with iron under acidic condition. This complex has a maximum absorption peak at 540 nm.
Self Provided：
Spectrophotometer, Water Bath, Desk Centrifuge, Adjustable Pipette, 1 mL Glass Cuvette, Mortar/Homogenizer/Cell Ultrasonic Crusher, Ice and Distilled Water.
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