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Determination of Significance：
Fructose 1,6-bisphosphatase(FBP) also known as fructose-1,6-diphosphatase, which plays a key role in the gluconeogenesis and the synthesis of photosynthetic assimilate sucrose.
Measurement Principle：
FBP catalyzes fructose 1,6-diphosphate and water to produce 6-phosphate fructose and inorganic phosphorus. Glucose-phosphate isomerase and 6-glucose-phosphate dehydrogenase added to the reaction system that catalyze the formation of 6-glucose-phosphate gluconic acid and NADPH in turn. In this kit, the activity of FBP is determined by the increase rate of NADPH at 340 nm.
Self Provided：
Ultraviolet spectrophotometer, desk centrifuge, adjustable pipette, water bath, 1 mL quartz cuvette, mortar/homogenizes, ice and distilled water.
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