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Citrate Synthase(CS) Activity Assay Kit

Storage:Store at -20℃,6 months

Citrate Synthase(CS) Activity Assay Kit
Cat.No:
BC1060
Brand:
Solarbio

Determination of Significance:

Citrate Synthase (CS, EC 2.3.3.1) is widely exists in animals, plants, microorganism and mitochondrial matrix of cultured cells. It is the first rate-limiting enzyme in the tricarboxylic acid cycle and one of the main regulatory sites.

Measurement Principle:

CS catalyzes acetyl CoA and acetoacetic acid to generate citryl coenzyme A, further hydrolysis to produce citric acid. The reaction promoted the transformation of colorless DTNB to yellow TNB, which has absorption at 412 nm.

Self Provided:

Spectrophotometer, low temperature centrifuge, water bath, mortar/homogenizer, adjustable pipette, 1 mL glass cuvette and distilled water.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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