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Pyruvate Decarboxylase(PDC) Activity Assay Kit

Storage:Store at -20℃,6 months

Pyruvate Decarboxylase(PDC) Activity Assay Kit
Cat.No:
BC1070
Brand:
Solarbio

Determination of Significance:

Pyruvate Decarboxylase (PDC) exists in yeast mainly, which is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylation to form acetaldehyde.

Measurement Principle:

Ethanol dehydrogenase (ADH) is added to further catalyze the reduction of acetaldehyde by NADH to produce ethanol and NAD+. NADH has a absorbance at 340 nm but NAD + not, the activity of PDC can be calculated by measuring decrease rate of absorption at 340 nm.

Self Provided:

Spectrophotometer, Water-Bath/Constant Temperature Incubator, Desk Centrifuge, Adjustable Pipette, 1 mL Quartz Cuvette, Mortar/Homogenizer/Cell ultrasonic Crusher, Ice and Distilled Water.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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