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Determination of Significance：
XOD (EC 1.17.3.2) catalyzes the oxidation of hypoxanthine to xanthine and superoxide anion, which is one of the main sources of active oxygen and is also one of the key enzymes of nucleotide metabolism. XOD is mainly distributed in mammalian heart, lung, liver and other tissues. When liver function impaired, XOD is released into serum in a large amount, which has specific significance for the diagnosis of liver damage.
Measurement Principle：
XOD catalyzes hypoxanthine to produce xanthine and superoxide anion. Superoxide anion reacts with hydroxylamine hydrochloride to form NO2-, and the NO2- under the action of p-aminobenzenesulfonamide and naphthalene ethylenediamine hydrochloride is produced a red azo compound with a characteristic absorption peak at 530 nm. The color depth is linear with XOD activity.
Self Provided：
Spectrophotometer, constant temperature foster box/water-bath, centrifuge, 1mL glass cuvette, adjustable transferpettor, mortar/homogenizer, ice and distilled water.
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