Determination of Significance:
Creatine kinase (CK) (EC 2.7.3.2) is also known as creatine phosphokinase, which mainly exists in heart, muscle and brain. It can reversibly catalyze the trans-phosphoryl reaction between creatine and ATP. It is an important kinase directly related to cell energy transport, muscle contraction and ATP regeneration.
Measurement Principle:
CK catalyzes creatine phosphate and ADP to generate creatine and ATP, hexokinase catalyzes ATP and glucose to generate glucose-6-phosphate, and glucose-6-phosphate dehydrogenase catalyzes glucose-6-phosphate and NADP+ to generate NADPH, resulting in an increase of 340 nm light absorption value, which is used to express CK enzyme activity.
Self Provided:
Scales, low temperature centrifuge, constant temperature water bath, spectrophotometer, 1 mL quartz cuvette and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.