Glutathione Reductases(GR) Activity Assay Kit
Cat.No:BC1160 Solarbio
Detection Method:Spectrophotometer
Storage:Store at 2-8℃,6 months
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Glutathione Reductases(GR) Activity Assay KitDetection Method:Spectrophotometer
Storage:Store at 2-8℃,6 months
Qty:
Size:
Name | Glutathione Reductases(GR) Activity Assay Kit |
Detection Method | Spectrophotometer |
Storage | Store at 2-8℃,6 months |
Unit | Kit |
Specification | 50T/48S |
Determination of Significance:
GR is a flavor-protein oxidoreductase widely existing in eukaryotes and prokaryotes. GR catalyzes the reduction of GSSG to GSH, which is one of the key enzymes of glutathione redox cycle (GR is usually replaced by TrxR in insects). GR catalyzes the reduction of GSSG to generate GSH by NADPH, which is helpful to maintain the body's GSH/GSSG ratio. GR plays a key role in the scavenging of reactive oxygen species in oxidative stress. In addition, GR also participates in the cycle pathway of ascorbic acid and glutathione.
Measurement Principle:
GR catalyzes the reduction of GSSG by NADPH to produce GSH, at the same time, NADPH dehydrogenation produces NADP+. NADPH has a characteristic absorption at 340 nm. On the contrary, NADP+ has no absorption peak at this wavelength. The rate of NADPH dehydrogenation is determined by measuring the rate of decrease of absorbance at 340 nm, thereby calculating GR activity.
Self Provided:
Spectrophotometer,low temperature centrifuge, water bath,adjustable pipette, 1 mL quartz cuvette and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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