Determination of Significance:
AAO is a glycoprotein that located in plant cell wall, belong to the "blue copper oxidase" family. Ascorbic acid and AAO in the cell wall are closely related to cell wall metabolism and growth. AAO catalyzes the oxidation of AsA to MDHA, which can be reduced by cytochrome b on the plasma membrane. The transmembrane transport of electrons in this process can promote cell growth.
Measurement Principle:
AAO can directly oxidize AsA. The activity of AAO can be calculated by measuring the oxidation amount of AsA.
Self Provided:
Low temperature centrifuge, ultraviolet spectrophotometer, 1 mL quartz cuvette, adjustable pipette, mortar, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.