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Determination of Significance：
Hydroxyl radical is a kind of free radical produced by human body in the course of metabolism, which is highly toxic and harmful to organisms. It can cause oxidative damage to carbohydrates, amino acids, proteins and nucleic acids in tissues, leading to cell necrosis or mutation. Hydroxyl radical scavenging capacity is one of the important indicators of antioxidant capacity of samples. It has been widely used in the research of antioxidant health products and medicines.
Measurement Principle：
H2O2/Fe2+ generates hydroxyl radicals through Fenton reaction, and oxidizes Fe2+ into Fe3+ in the aqueous reagent of phenanthroline-Fe2+, resulting in the decreased absorbance of 536 nm, and the inhibition of the decreased rate of absorbance of 536 nm, reflecting the ability of scavenging hydroxyl radicals of samples.
Self Provided：
Spectrophotometer, 1mL glass cuvette, balance, centrifuge, water bath/constant temperature foster box, transferpettor, mortar/homogenizer, ice and distilled water.
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