Determination of Significance:
Flavonoids are a class of poly-phenyl compounds, which are plant secondary metabolites. They have the advantages of anti-inflammatory, antibacterial, hypolipemic, scavenging hydroxyl free radicals and cancer prevent.
Measurement Principle:
In the alkaline nitrite solution, the flavonoid and the aluminum ion can form a red complex with a characteristic absorption peak at 470 nm. The sample flavonoid content can be calculated by measuring the absorbance of the sample extract at 470 nm.
Self Provided:
Spectrophotometer, balance, oven, 30-50 mesh sieve, comminution apparatus, centrifuge, 1mL glass cuvette, 60% ethanol, distilled water, ultrasonic cleaner, constant temperature incubator.
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