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Determination of Significance：
The sulfhydryl mainly includes glutathione sulfhydryl group and protein sulfhydryl group in vivo. The former can not only repair the oxidative damage protein, but also participate in scavenging the reactive oxygen species. The latter plays an important role in maintaining the protein conformation. The content of protein sulfhydryl can be determined indirectly by measuring the content of total sulfhydryl and GSH.
Measurement Principle：
Sulfhydryl react with 5,5’-dithio-bis-nitrobenzoic acid (DTNB) to form yellow compound which has max absorbance peak at 412 nm.
Self Provided：
Spectrophotometer, 1 mL glass cuvette, balance, centrifuge, constant temperature water bath, motor/homogenizer, and distilled water.
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