Determination of Significance:
GLS (EC3.5.1.2) is mainly found in higher animals and some bacteria and plant roots, catalyzing the hydrolysis of glutamine into glutamic acid and ammonia, which plays an important role in the regulation of nitrogen metabolism, especially the regulation of free ammonia and urea metabolism.
Measurement Principle:
The kit uses the indophenol blue colorimetric method to determine ammonia produced by glutamine of GLS-catalyzed to indicate activity
Self Provided:
Spectrophotometer, adjustable pipette, mortar/homogenizer, centrifuge, 1 mL glass cuvette, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:
These protocols are for reference only. Solarbio does not independently validate these
methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.