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Determination of Significance：
GPT is widely found in animals, plants, microbes and cultured cells, which is an important enzyme in amino acid metabolism. It catalyzes the transamination of amino acid and keto acid. In addition, GPT activity is very high in Mammalian liver cells. GPT is released into the blood when liver cells necrotic, serum GPT activity is significantly increased. Therefore, GPT is recommended as the most sensitive indicator of liver damage by the World Health Organization.
Measurement Principle：
GPT catalyzes the transamination reaction of alanine and α-ketoglutarate to generate pyruvate and glutamic acid; the addition of 2,4-dinitrophenylhydrazine solution not only terminates the above reaction, but also increases Into phenylpyrene pyruvate; which shows brownish red in alkaline condition, the activity of GOT enzyme activity can be calculated by measuring the absorbance of 505 nm.
Self Provided：
Spectrophotometer, water bath, 1 mL glass cuvette, desk centrifuge, transferpettor, distilled water, ice and mortar/homogenizer.
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