Soluble Starch Synthase(SSS) Activity Assay Kit
Cat.No:BC1850 Solarbio
Detection Method:Spectrophotometer
Storage:Store at -20℃,6 months
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Soluble Starch Synthase(SSS) Activity Assay KitDetection Method:Spectrophotometer
Storage:Store at -20℃,6 months
Qty:
Size:
Name | Soluble Starch Synthase(SSS) Activity Assay Kit |
Detection Method | Spectrophotometer |
Storage | Store at -20℃,6 months |
Unit | Kit |
Specification | 25T/24S 50T/48S |
Determination of Significance:
Soluble Starch Synthase (SSS, EC 2.4.1.21) usually present in the free matrix in the plastid matrix, which catalyzes the elongation of the starch chain, mainly responsible for the synthesis of amylopectin.
Measurement Principle:
SSS catalyzes the reaction of ADPG with starch primer (glucan), transfers glucose molecules to starch primers, and simultaneously produces ADP. Add pyruvate kinase, hexokinase and 6-phosphate glucose dehydrogenase to the reaction system. These enzymes in turn catalyze NADP+ reduction to NADPH, the amount of NADPH produced is proportional to the amount of ADP produced in the previous step reaction, and the SSS activity can be calculated by measuring the increase of NADPH at 340 nm.
Self Provided:
Ultraviolet Spectrophotometer, Water Bath, Desk Centrifuge, Transferpettor, 1 mL Quartz Cuvette, Mortar/Homogenizer, Ice and Distilled Water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Excessive nitrogen in field-grown rice suppresses grain filling of inferior spikelets by reducing the accumulation of cytokinin and auxin
Click to check >>Author:陈银科
IF:5.2000
Publish_to:Field Crops Research
PMID: