Determination of Significance:
Total cholesterol (TC) is the sum of cholesterol contained in all lipoproteins, including free cholesterol and cholesteryl esters.
Measurement Principle:
The enzyme esterase catalyzes the hydrolysis of cholesteryl esters to produce free cholesterol (FC) and free fatty acids (FFA), thus converting cholesteryl esters to FC; furthermore, cholesteryl oxidase catalyzes the oxidation of FC to produce 4-Cholestenone and H2O2; finally, peroxidase catalyzes the oxidation of 4-aminoantipyrine and phenol by H2O2 to produce red quinones, which have a characteristic absorption peak at 500 nm. The color shade is proportional to the TC content.
Self Provided:
Spectrophotometer, balance, low temperature table centrifuge, water-bath, 1 mL glass cuvette, pipette, EP tube, distilled water, isopropyl alcohol.
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