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Triose Phosphate Isomerase (TPI) Activity Assay Kit

Storage:Store at -20℃,6 months

Triose Phosphate Isomerase (TPI) Activity Assay Kit
Cat.No:
BC2260
Brand:
Solarbio

Determination of Significance:

Triose phosphate isomerase is an important glycolytic enzyme, which is widely present in animals, plants and microorganisms. Triose phosphate isomerase participates in glucose metabolism in the body. Triose phosphate isomerase plays an important role in glycolysis, gluconeogenesis, fatty acid biosynthesis and pentose phosphate metabolism.

Measurement Principle:

Triose phosphate isomerase converts dihydroxyacetone phosphate to glyceraldehyde 3-phosphate. Glyceraldehyde 3-phosphate and NAD generate 3-phosphoglycerate and NADH under the action of glyceraldehyde 3-phosphate dehydrogenase. The change in absorbance at 340nm reflects the activity of triose phosphate isomerase.

Self Provided:

Ultraviolet spectrophotometer, balance, water-bath, centrifuge, transferpettor, 1mL quartz cuvette, mortar/homogenizer, ice and distilled water.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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