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Determination of Significance：
ACP is an enzyme that catalyzes the hydrolysis of proteins in acidic environments. The enzyme is mainly used in alcohol fermentation, beer brewing, fur softening, fruit wine clarification, soy sauce brewing, feed and so on.
Measurement Principle：
In acidic condition, ACP can catalyzes the hydrolysis of casein to produce tyrosine. In alkaline condition, tyrosine reduces phosphomolybdic acid compound to tungsten blue which has a characteristic absorption peak at 680 nm, and the activity of ACP is calculated by measuring its absorbance increase.
Self Provided：
Mortar/homogenizer, desk centrifuge, spectrophotometer, water bath, magnetic stirrer, transferpettor, 1.5 mL centrifuge tube, 1 mL glass cuvette and distilled water.
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