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Determination of Significance：
NP catalyze the hydrolysis of protein under certain temperature and neutral PH conditions. It has the characteristics of safety, non-toxicity, strong hydrolysis ability and wide range of action. So NP is often used in the production of food, feed, cosmetics and nutritional health products.
Measurement Principle：
In neutral conditions, NP can catalyze the hydrolysis of casein to produce tyrosine. In alkaline condition, tyrosine reduces phosphomolybdic acid compounds to tungsten blue, which has a characteristic absorption peak at 680 nm.
Self Provided：
Mortar/homogenizer, desk centrifuge, spectrophotometer, water bath, magnetic stirrer, transferpettor, 1.5 mL centrifuge tube, 1 mL glass cuvette and distilled water.
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