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My CartReactivity：Fusion proteins containing the Flag Tag
Application：ELISA,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Host：Rabbit
Qty：
Size：
| Name | Anti-FLAG Tag Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:5000-20000. ELISA 1:5000-10000. |
| Target | FLAG Tag |
| Background | The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins. |
| Immunogen | A synthetic peptide |
| Calculated MW | 55kDa |
| Purification Method | Affinity purification |
| Storage Buffer | pH7.4 PBS，0.05% NaN3，40% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:5000-20000. ELISA 1:5000-10000 |
| Specification | 50ul 100ul |
| Reactivity | Fusion proteins containing the Flag Tag |
| Application | ELISA WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with FLAG Tag antibody diluted at 1:5000;Lane: Recombinant protein.
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Purification of the insecticidal Cry2Ad protein from a Bt-isolated BRC-HZP10 strain and its toxin assay to the diamondback moth, Plutella xylostella (L.)
Click to check >>Author：J.Y. Liao, Y.Q. Gao, Q.Y. Wu, Y.C. Zhu and M.S. Yo
IF：0.5800
Publish_to：Genetics and Molecular Research
PMID：26214446
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