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Determination of Significance：
Pectinase is one of the enzymes that decompose pectin, including protopectinase, pectinesterase, polygalacturonase and pectinase. It widely exists in fruits of higher plants and microorganisms and is the most important enzyme in fruit processing.
Measurement Principle：
Pectinase hydrolyzes pectin to produce galacturonic acid, which reacts with DNS reagent to produce brownish red substance with characteristic absorption peak at 540 nm. The activity of pectinase can be calculated by measuring the change of absorption value at 540 nm.
Self Provided：
Spectrophotometer, centrifuge, water bath, 1 mL glass cuvette, adjustable pipette, mortar/homogenizer, ice and distilled water.
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