Mitochondrial complex III / CoQ-cytochrome C reductase Activity Assay Kit
Cat.No：BC3240 Solarbio
Detection Method：Spectrophotometer
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Energy Metabolism Series > Mitochondrial Respiratory Chain Series > Mitochondrial complex III / CoQ-cytochrome C reductase Activity Assay Kit
Product Information
| Name | Mitochondrial complex III / CoQ-cytochrome C reductase Activity Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 25T/12S 50T/24S |
Determination of Significance：
Mitochondrial complex III (EC 1.10.2.2), also known as CoQ-cytochrome C reductase, is widely found in mitochondria of animals, plants, microorganisms and cultured cells. It is a common component of the main pathway and branch of the mitochondrial respiratory electron transport chain. Mitochondrial complex III is responsible for transferring hydrogen from reduced CoQ to cytochrome C and then produce reduced cytochrome C.
Measurement Principle：
Unlike oxidized cytochrome C, reduced cytochrome C has a characteristic absorption at 550 nm, so the rate of increase in light absorption at 550 nm can reflect mitochondrial complex III enzyme activity.
Self Provided：
Spectrophotometer, water bath, desk centrifuge, transferpettor, mortar/homogenizer/cell ultrasonic crusher, 1ml glass cuvette, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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