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Storage:Store at -20℃，6 months
Determination of Significance：
UDP-glucose pyrophosphorylase (UDP-glucose pyrophosphosphprylase, UGP, EC2.7.7.9) is widely distributed in nature. It catalyzes the activation of glucose before glycogen synthesis. UDP-glucose (UDPG) is synthesized from glucose-1-phosphate and UTP. UDPG is the main active enzyme form in higher plants and animals. As a glucose-based donor, it participates in the synthesis and metabolism of glycogen, sucrose, cellulose, etc.
Measurement Principle：
UGP can catalyze the reversible formation of glucose-1-phosphate. NADP was transformed into NADPH by phosphoglucose mutase and 6-phosphoglucose dehydrogenase. UGP activity can be reflected by the change of 340nm absorption value.
Self Provided：
Ultraviolet spectrophotometer, balance, low temperature desk centrifuge, water-bath, transferpettor, 1 mL quartz cuvette, EP tube, mortar/homogenizer, ice and distilled water.
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