English Name |
Anti-HSPA5 Polyclonal Antibody |
Chinese Name |
葡萄糖调节蛋白78/热休克蛋白70蛋白5抗体 |
Target |
HSPA5 |
Synonym |
BIP, HSPA5, GRP-78, glucose regulated protein 78, 78 kDa glucose regulated protein, BIP, Endoplasmic reticulum lumenal Ca2+ binding protein grp78, FLJ26106, Glucose Regulated Protein 78kDa, GRP 78, Heat Shock 70kDa Protein 5, HSPA 5, HSPA-5, Immunoglobulin Heavy Chain Binding Protein, MIF2, GRP78_HUMAN |
Backgroud |
The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. |
Swiss Prot |
P11021 |
Gene ID |
3309 |
Subcellular Localization |
Cytoplasm Endoplasmic reticulum lumen Melanosome |
Host |
Rabbit |
Clone Type |
Polyclonal Antibody |
Subtype |
IgG |
Protein MW |
72kDa |
Dilution Ratio |
WB 1:2000-5000. IHC 1:50-200. IF 1:50-200. |
Immunogen |
A synthetic peptide of human HSPA5 |
Public Immunogen Range |
260-310/654aa |
Purification Method |
Affinity purification |
Storage Buffer |
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
Storage |
Store at -20℃, avoid freeze/thaw cycles. |
Unit |
Piece |
Cross-Reactivity |
Human Rat Mouse |
Application |
WB IHC IF |
Specification |
50ul 100ul |