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My CartReactivity：Human,Rat,Mouse
Application：WB,IHC,ELISA
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：3312
Host：Rabbit
Qty：
Size：
| Name | Anti-HSPA8 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Cow;Monkey;Hamster;Chimpanzee;Horse;Chicken |
| Dilution Ratio | WB 1:8000-12000. IHC 1:100-300. |
| Target | HSPA8 |
| Background | This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Swiss Prot | P11142 |
| Gene ID | 3312 |
| Subcellular Location | Cell membrane Cytoplasm Melanosome Nucleus nucleolus |
| Immunogen | A synthetic peptide of human HSPA8 |
| Public Immunogen Range | 510-560/646aa |
| Calculated MW | 71/54kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:8000-12000. IHC 1:100-300. ELISA 1:8000-80000. |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | IHC WB ELISA |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with HSPA8 antibody diluted at 1:10000;Lane: A431,HeLa,Raw264.7,293T,Mouse brain.
Immunohistochemistry of paraffin-embedded Human stomach cancer with HSPA8 antibody diluted at 1:200.
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