{{cart_num}}
My CartApplication：WB,IP,IF
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Mouse
Qty：
Size：
| Name | Anti-Flag tag Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 2C5 |
| Dilution Ratio | WB 1:10000-15000. IP 1:100-300. IF 1:500-3000. |
| Target | Flag tag |
| Background | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. IF there is no against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an against the Flag sequence. |
| Immunogen | A synthetic peptide coupled to KLH |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Application | IP IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with Flag tag antibody diluted at 1:10000;Lane: Recombinant protein.
Immunofluorescence analysis of 293T cells using Flag tag antibody diluted at 1:2000.
Sorry, there is no more information.
Fish-specific Toll-like receptor 14 (TLR14) from Asian swamp eel (Monopterus albus) is involved in immune response to bacterial infection
Click to check >>Author：Liu R
IF：4.5810
Publish_to：Fish & Shellfish Immunology
PMID：35421574
Phosphorylation of SiRAV1 at Ser31 regulates the SiCAT expression to enhance salt tolerance in Setaria italica
Click to check >>Author：Qiao-lu LI, Zhi-yong LI, Meng-meng WANG, Jing-wei YAN, Lin FANG
IF：4.3840
Publish_to：Journal of Integrative Agriculture
PMID：
Effects of ELL-associated factor 2 on ultraviolet radiation-induced cataract formation in mice
Click to check >>Author：Yanhua Jiang, Rongrong Fu, Jiangyue Zhao, Di Wu, G
IF：1.9220
Publish_to：Molecular Medicine Reports
PMID：26328919
Manual Download
