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My CartReactivity：Human
Application：IF,IHC,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：7157
Host：Mouse
Qty：
Size：
| Name | Anti-P53 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 6C4 |
| Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. IF 1:100-200. IP 1:50-500. |
| Target | TP53 |
| Background | p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. |
| Swiss Prot | P04637 |
| Gene ID | 7157 |
| Subcellular Location | Cytoplasm Endoplasmic reticulum Mitochondrion matrix Nucleus PML body |
| Immunogen | A synthetic peptide coupled to KLH |
| Calculated MW | 44/23/33/34/38kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human |
| Application | WB IHC IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with P53 antibody diluted at 1:2000;Lane: 293T.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with P53 antibody diluted at 1:100.
Immunofluorescence analysis of Hela cells using TP53 antibody diluted at 1:100.
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