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My CartReactivity：Human,Rat,Mouse
Application：IHC;WB;IP
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：3855
Host：Mouse
Qty：
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| Name | Anti-KRT7 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 12D7 |
| Predicted Application | IF |
| Dilution Ratio | WB 1:2000-5000. IHC 1:100-300. IP 1:100-2000. |
| Target | KRT7 |
| Background | Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19. |
| Swiss Prot | P08729 |
| Gene ID | 3855 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide coupled to KRT7 |
| Calculated MW | 51kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | IHC;WB;IP |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with KRT7 antibody diluted at 1:3000;Lane: HeLa,A549,Mouse kidney.
Immunohistochemistry of paraffin-embedded Human endometrial cancer with KRT7 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human breast cancer with KRT7 antibody diluted at 1:100.
Immunoprecipitation analysis of Hela extracts using KRT7 antibody(IP:1:2000;WB:1:3000).Control IgG: Normal Mouse IgG.
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