Product introduction

Fungal or yeast extracts contain many endogenous proteases, phosphatases, etc., which easily lead to the degradation or demodification of proteins in the extracts, thus affecting the subsequent protein detection. Therefore, adding appropriate inhibitors such as protease and phosphatase to the extracts is an effective way to prevent protein degradation and demodification. This product is used in a mixture of protease inhibitors derived from fungal or yeast proteins, containing a broad spectrum of serine, cysteine and acid protease inhibitors, as well as metalloprotease inhibitors.

Scope of application

"It inhibits various protease activities in fungal or yeast extracts, such as serine proteases, cysteine proteases, threonine and aspartic proteinases, metalloproteinases, etc." It is suitable for Western Blot and co-immunoprecipitation detection of phosphorylated proteins, protein kinase activity determination, etc.

Usage method

Protease inhibitor mixture (fungal or yeast extract, 100X), each added to the lysate at a ratio of 1:100, mixed and ready to use. The lysates containing the protease phosphatase inhibitor mixture should be prepared on the spot, and should not be prepared and frozen for later use.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.