English
中文
Manual Download
Product introduction
Bacterial extracts contain many endogenous proteases, phosphatases, etc., which easily lead to the degradation or demodification of proteins in the extracts, thus affecting the subsequent protein detection. Therefore, adding appropriate inhibitors such as protease and phosphatase to the extracts is an effective way to prevent protein degradation and demodification. This product is a cocktail of protease inhibitors optimized and tested for bacterial protein extraction and contains a broad spectrum of serine, cysteine and acid protease inhibitors, as well as aminopeptidase inhibitors.
Scope of application
It inhibits various protease activities in bacterial extracts, such as serine protease, aminopeptidase, cysteine protease, threonine and aspartic protease, metalloproteinase, etc.
Usage method
1. Protease inhibitor mixture (for bacterial extraction, 100X) is added to the lysate at a ratio of 1:100 when used, and can be used after mixing. The lysates containing the mixture of protease phosphatase inhibitors should be prepared on the spot, and should not be prepared and frozen for later use.
2, 0.1M EDTA was also added to the lysate at a ratio of 1:100 as needed.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
