Protease Inhibitor Cocktail for Bacterial Cell Extracts, 100X

Product introduction

Bacterial extracts contain many endogenous proteases, phosphatases, etc., which easily lead to the degradation or demodification of proteins in the extracts, thus affecting the subsequent protein detection. Therefore, adding appropriate inhibitors such as protease and phosphatase to the extracts is an effective way to prevent protein degradation and demodification. This product is a cocktail of protease inhibitors optimized and tested for bacterial protein extraction and contains a broad spectrum of serine, cysteine and acid protease inhibitors, as well as aminopeptidase inhibitors.

Scope of application

It inhibits various protease activities in bacterial extracts, such as serine protease, aminopeptidase, cysteine protease, threonine and aspartic protease, metalloproteinase, etc.

Usage method

1. Protease inhibitor mixture (for bacterial extraction, 100X) is added to the lysate at a ratio of 1:100 when used, and can be used after mixing. The lysates containing the mixture of protease phosphatase inhibitors should be prepared on the spot, and should not be prepared and frozen for later use.

2, 0.1M EDTA was also added to the lysate at a ratio of 1:100 as needed.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.