Protease Inhibitor Cocktail for Purification of His-Tagged Proteins, 100X
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Oscillating Mixing & Stirring & Dispersing Instrument
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> Protein and Immunity > Protein Extraction/Quantification > Protein Extraction Reagent > Protease Inhibitor Cocktail for Purification of His-Tagged Proteins, 100X
Product Information
| Gene Name | Box |
| Specification | 100T |
Product introduction
Cell or tissue extracts contain many endogenous proteases, phosphatases, etc., which easily lead to the degradation or demodification of proteins in the extracts, thus affecting the subsequent protein detection. Therefore, adding appropriate inhibitors such as protease and phosphatase to the extracts is an effective way to prevent protein degradation and demodification. Protease inhibitor cocktail for His-Tag protein purification, including a broad spectrum of serine, cysteine and acid protease inhibitors, as well as aminopeptidase inhibitors.
Scope of application
It inhibits various protease activities in His-Tag protein purification, such as serine protease, aminopeptidase, cysteine protease, threonine and aspartic protease, and metalloproteinase.
Usage method
The protease inhibitor mixture (for bacterial extraction, 100X) was added to the lysate at a ratio of 1:100 and mixed before use. The lysates containing the protease phosphatase inhibitor mixture should be prepared on the spot, and should not be prepared and frozen for later use.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Dabie bandavirus infection induces macrophagic pyroptosis and this process is attenuated by platelets
Click to check >>Author：Sicong Yu, Qinyi Zhang, Lingxuan Su, Ji He, Wen Shi, Hao Yan, Haiyan Mao, Yi Sun, Dongqing Cheng, Xuan Wang, Yanjun Zhang, Lei Fang
IF：3.8000
Publish_to：PLoS Neglected Tropical Diseases
PMID：37486928
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