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Product introduction
Cell or tissue extracts contain many endogenous proteases, phosphatases, etc., which easily lead to the degradation or demodification of proteins in the extracts, thus affecting the subsequent protein detection. Therefore, adding appropriate inhibitors such as protease and phosphatase to the extracts is an effective way to prevent protein degradation and demodification. Protease inhibitor cocktail for His-Tag protein purification, including a broad spectrum of serine, cysteine and acid protease inhibitors, as well as aminopeptidase inhibitors.
Scope of application
It inhibits various protease activities in His-Tag protein purification, such as serine protease, aminopeptidase, cysteine protease, threonine and aspartic protease, and metalloproteinase.
Usage method
The protease inhibitor mixture (for bacterial extraction, 100X) was added to the lysate at a ratio of 1:100 and mixed before use. The lysates containing the protease phosphatase inhibitor mixture should be prepared on the spot, and should not be prepared and frozen for later use.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
