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My CartReactivity：Human,Mouse
Application：IHC,ELISA,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：2735
Host：Rabbit
Qty：
Size：
| Name | Anti-GLI1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. IHC 1:50-200. ELISA 1:5000-10000. |
| Target | GLI1 |
| Background | This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Swiss Prot | P08151 |
| Gene ID | 2735 |
| Subcellular Location | Nucleus |
| Immunogen | A synthetic peptide of human GLI1 |
| Calculated MW | 118kDa |
| Purification Method | Affinity purification |
| Storage Buffer | pH7.4 PBS，0.05% NaN3，40% Glycerol. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Mouse |
| Application | ELISA WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Human prostate cancer with GLI1 antibody diluted at 1:100.
Western blot analysis with GLI1 antibody diluted at 1:1000;Lane: Raji,HepG2,PC-3M-IE8,HT29.
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