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My CartReactivity：Human,Mouse
Application：WB,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：219333
Host：Rabbit
Qty：
Size：
| Name | Anti-USP12 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000.IHC 1:25-100. |
| Target | USP12 |
| Background | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP12 (ubiquitin specific peptidase 12), also known as UBH1 or USP12L1, is a 370 amino acid protein belonging to the peptidase C19 family and the USP12/USP46 subfamily. Considered a deubiquitinating enzyme, it is suggested that USP12 has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have high activity. The gene encoding USP12 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. |
| Swiss Prot | O75317 |
| Gene ID | 219333 |
| Subcellular Location | Cytoplasm Cell membrane |
| Immunogen | Recombinant protein of human USP12 |
| Calculated MW | 43kDa |
| Purification Method | Affinity purification |
| Storage Buffer | pH7.4 PBS，0.05% NaN3，40% Glycerol. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:500-2000. IHC 1:25-100. |
| Specification | 50ul 100ul |
| Reactivity | Human Mouse |
| Application | IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Human pancreatic cancer with USP12 antibody diluted at 1:50.
Western blot analysis with USP12 antibody diluted at 1:1000;Lane: Mouse liver.
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