C4:0 METHYL BUTYRATE 2.63%;

C6:0 METHYL HEXANOATE 2.63%;

C8:0 METHYL OCTANOATE 2.63%;

C10:0 METHYL DECANOATE 2.63%;

C11:0 METHYL UNDECANOATE 2.63%;

C12:0 METHYL LAURATE 2.63%;

C13:0 METHYL TRIDECANOATE 2.63%;

C14:0 METHYL MYRISTATE 2.63%;

C14:1 METHYL MYRISTOLEATE 2.63%;

C15:0 METHYL PENTADECANOATE 2.63%;

C15:1 METHYL 10-PENTADECENOATE 2.63%;

C16:0 METHYL PALMITATE 5.26%;

C16:1 METHYL PALMITOLEATE 2.63%;

C17:0 METHYL HEPTADECANOATE 2.63%;

C17:1 METHYL 10-HEPTADECENOATE 2.63%;

C18:0 METHYL STEARATE 2.63%;

C18:1 METHYL OLEATE 2.63%

;C18:1T METHYL ELAIDATE 2.63%;

C18:2 METHYL LINOLEATE 2.63%;

C18:2T METHYL LINOELAIDATE 2.63%;

C18:3 METHYL LINOLENATE 2.63%;

C18:3 METHYL GAMMA LINOLENATE 2.63%;

C20:0 METHYL ARACHIDATE 2.63%;

C20:1 METHYL 11-EICOSENOATE 2.63%;

C21:0 METHYL HENEICOSANOATE 2.63%;

C20:2 METHYL 11-14 EICOSADIENOATE 2.63%;

C22:0 METHYL BEHENATE 2.63%;

C22:1 METHYL ERUCATE 2.63%;

C20:3 METHYL 11-14-17 EICOSATRIENOATE 2.63%;

C20:3 METHYL HOMOGAMMA LINOLENATE 2.63%;

C20:4 METHYL ARACHIDONATE 2.63%;

C23:0 METHYL TRICOSANOATE 2.63%;

C22:2 METHYL DOCOSADIENOATE 2.63%;

C24:0 METHYL LIGNOCERATE 2.63%;

C20:5 METHYL EICOSAPENTAENOATE 2.63%;

C24:1 METHYL NERVONATE 2.63%;

C22:6 METHYL DOCOSAHEXAENOATE 2.63%.

Examples of using this product（for reference only）

UPLC–Q-Exactive Focus MS/MS

C18 column (100 mm × 2.1 mm, 1.7 μm) and coupled to an UPLC-Q-Exactive Focus mass spectrometer with electrospray ionization source in both positive (POS) and negative (NEG) modes was used to determine the lipid profile of IPO. The injection volume was 1 μl, and the column temperature was maintained at 50℃. Gradient elution was carried out with acetonitrile/water (60:40 [v/v], 0.1% formic acid with 10 mmol/L ammonium formate) (solvent A) and isopropanol/acetonitrile (90:10 [v/v], 0.1% formic acid with 10 mmol/L ammonium formate) (solvent B) at a flow rate of 0.25 ml/min. An increasing linear gradient of solvent A (v/v) was used as follows: 0–5 min, 70%?57% A; 5–5.1 min, 57%?50% A; 5.1–14 min, 50%?30% A; 14–14.1 min, 30% A; 14.1–21 min, 30%?1% A; 21–24 min, 1% A; 24–24.1 min, 1%?70% A; 24.1–28 min, 70% A. Mass spectrometry conditions were as follows: spray voltage, 3.5 kV (POS) and ?2.5 kV (NEG); capillary temperature, 325℃; mass range, m/z 150–2000; normalized collision energy, 30 eV; sheath gas, 30 psi; auxiliary gas, 10 psi. Data-dependent acquisition MS/MS experiments were performed with HCD scan.

References：

Wen L, Xiang X, Wang Z, Yang Q, Guo Z, Huang P, Mao J, An X, Kan J. Evaluation of cultivars diversity and lipid composition properties of Idesia polycarpa var. vestita Diels. J Food Sci. 2022 Sep;87(9):3841-3855. doi: 10.1111/1750-3841.16293. Epub 2022 Aug 21. PMID: 35988127.