CAS:111-02-4
Appearance:Colorless to light yellow liquid
Storage:2-8℃
Purity:GC≥98%
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HPLC
The products were purified using the XDB-C18 separationcolumn (5 μm, 250 × 4.6 mm). Retinol and retinal were detected in a mobile phase consistingof methanol and acetonitrile in a ratio of 95:5 at the detection temperature of 25 ℃ with thedetection wavelength of 352 nm. The mobile phase consisted of methanol, acetonitrile, andisopropanol in a ratio of 3:5:2, and the detection temperature was 40 ℃. The detectionwavelength used for β-carotene was 450 nm.Squalene was detected at 210 nm usingmobile phase methanol at 40 ℃.
References:
Wang X, Xu X, Liu J, Liu Y, Li J, Du G, Lv X, Liu L. Metabolic Engineering of Saccharomyces cerevisiae for Efficient Retinol Synthesis. J Fungi (Basel). 2023 Apr 26;9(5):512. doi: 10.3390/jof9050512. PMID: 37233223; PMCID: PMC10219262.
GC-MS
Gas chromatography in series with mass spectrometry (GC-MS) with a MS capillary column (0.25 mm, 30 m × 0.25 mm) was used to evaluate the treated samples. The following setup parameters were used: split injection ratio of 50:1, flow rate of 1.2 mL/min of helium, transfer line temperature of 250℃, ion source temperature of 230 ℃, and injector temperature of 280℃. After holding the column temperature at 200 ℃ for 0.5 min, it was increased to 300 ℃ at a rate of 10 ℃/min and maintained at this temperature for 20 min. The modified EI ionization mode ranged from 50 to 650.
References:
Ma Y, Wang G, Deng Z, Zhang B, Li H. Effects of Endogenous Anti-Oxidative Components from Different Vegetable Oils on Their Oxidative Stability. Foods. 2023 Jun 5;12(11):2273. doi: 10.3390/foods12112273. PMID: 37297517; PMCID: PMC10252449.
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