Squalene

Examples of using this product（for reference only）

HPLC

The products were purified using the XDB-C18 separationcolumn (5 μm, 250 × 4.6 mm). Retinol and retinal were detected in a mobile phase consistingof methanol and acetonitrile in a ratio of 95:5 at the detection temperature of 25 ℃ with thedetection wavelength of 352 nm. The mobile phase consisted of methanol, acetonitrile, andisopropanol in a ratio of 3:5:2, and the detection temperature was 40 ℃. The detectionwavelength used for β-carotene was 450 nm.Squalene was detected at 210 nm usingmobile phase methanol at 40 ℃.

References：

Wang X, Xu X, Liu J, Liu Y, Li J, Du G, Lv X, Liu L. Metabolic Engineering of Saccharomyces cerevisiae for Efficient Retinol Synthesis. J Fungi (Basel). 2023 Apr 26;9(5):512. doi: 10.3390/jof9050512. PMID: 37233223; PMCID: PMC10219262.

GC-MS

Gas chromatography in series with mass spectrometry (GC-MS) with a MS capillary column (0.25 mm, 30 m × 0.25 mm) was used to evaluate the treated samples. The following setup parameters were used: split injection ratio of 50:1, flow rate of 1.2 mL/min of helium, transfer line temperature of 250℃, ion source temperature of 230 ℃, and injector temperature of 280℃. After holding the column temperature at 200 ℃ for 0.5 min, it was increased to 300 ℃ at a rate of 10 ℃/min and maintained at this temperature for 20 min. The modified EI ionization mode ranged from 50 to 650.

References：

Ma Y, Wang G, Deng Z, Zhang B, Li H. Effects of Endogenous Anti-Oxidative Components from Different Vegetable Oils on Their Oxidative Stability. Foods. 2023 Jun 5;12(11):2273. doi: 10.3390/foods12112273. PMID: 37297517; PMCID: PMC10252449.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.