Determination of Significance:
Cellulose (CLL) is a straight chain polymer composed of β-D-glucose units linked by β-1,4-glycoside bond, which is usually combined with hemicellulose, pectin and lignin, and is the main structural component of plant cell wall. Cellulose products are widely used in food, paper, plastics, explosives, electrical engineering and scientific research equipment.
Measurement Principle:
Cellulose can be decomposed into β-D-glucose by heating in acid condition. Under the condition of strong acid, the content of cellulose is determined with anthrone chromogenic agent.
Self Provided:
Spectrophotometer/microplate reader, low temperature centrifuge, water-bath, micro glass cuvette/96 well flat-bottom plate, ransferpettor, mortar/homogenizer, ice, acetone, concentrated H2SO4, anhydrous ethanol, distilled water and EP tube.
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