Reactivity:Human IgG
Application: IF
Host:Rabbit
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Array ( [75] => Array ( [id] => 75 [name] => Secondary Antibodies [url] => category-75-b0.html [cat_desc] => [children] => Array ( [840] => Array ( [id] => 840 [name] => SAlexa Fluor-Conjugated [url] => category-840-b0.html [cat_desc] => ) [216] => Array ( [id] => 216 [name] => Other Fluorescent-Conjugated [url] => category-216-b0.html [cat_desc] => ) [276] => Array ( [id] => 276 [name] => Other-Conjugated [url] => category-276-b0.html [cat_desc] => ) [80] => Array ( [id] => 80 [name] => Colloidal Gold Reagent [url] => category-80-b0.html [cat_desc] => 胶体金是由氯金酸在还原剂的作用下还原成的金颗粒溶液。博士德的胶体金采用枸櫞酸三钠或枸櫞酸三钠+维生素C作为还原剂。通过改变还原剂的用量,就能获得一系列颗粒直径从5nm到15nm的胶体金。经过偶连、超速离心、密度梯度分层等步骤,生产出一系列直径的胶体金试剂。 ) [841] => Array ( [id] => 841 [name] => Alkaline Phosphatase(AP)-Conjugated [url] => category-841-b0.html [cat_desc] => ) [842] => Array ( [id] => 842 [name] => Colloidal Gold-Conjugated [url] => category-842-b0.html [cat_desc] => ) [81] => Array ( [id] => 81 [name] => FITC-Conjugated [url] => category-81-b0.html [cat_desc] => 索莱宝公司研制的FITC标记抗体,均为自己制备的免疫血清,免疫血清经过亲和层析的方法纯化出高纯度的IgG,采用直接标记法,将异硫氰酸荧光素(FITC,From Sigma,绿色荧光)共价交联到抗体IgG分子的赖氨酸基团上,并经过纯度、蛋白含量、FITC浓度及效价鉴定,为特异、灵敏和稳定的优质产品。 工作效价: 免疫荧光IF:1:50-500(以产品标签标识为准) ,最佳稀释度需根据样本抗原及一抗浓度而定。若用于其它实验,客户需要根据自己的实验摸索出最佳的稀释倍数,可用PBS(0.01mol/L, ) [78] => Array ( [id] => 78 [name] => Biotin-Conjugated [url] => category-78-b0.html [cat_desc] => 索莱宝公司研制的生物素化抗体是经亲和层析纯化成高纯度IgG成分,将进口的活化生物素(羟基琥珀酰亚胺酯,BNHS)共价交联到抗体IgG分子上制成。生物素化抗体标记的样品可应用酶标或荧光染料标记的亲和素或生物素-亲和素复合物来检测。本产品0.1ml含有0.2mg抗体,0.01M PBS, 2% BSA, 0.09% NaN3。 工作效价: ELISA法:1:500~1000 组织化学:1:50~100 免疫印迹:1:200~500。 具体效价以产品标签为准,最佳使用条件需根据所检测样本的抗原 ) [84] => Array ( [id] => 84 [name] => Purified Secondary Antibodies [url] => category-84-b0.html [cat_desc] => 纯化抗体本产品采用Protein A/G亲和层析纯化,制成高纯度(﹥95%)和高活性的纯化羊或兔IgG抗体。 本产品为PBS溶液,可根据用户要求,配成不同浓度,用前最好-20℃存放,可根据使用方法采用不同浓度稀释。 通常不同使用目的可分别用下列缓冲液稀释,注意完全溶解后充分混匀。冻存过久会产生少量聚合体,用前最好低温高速离心,去除冷聚合球蛋白及变性蛋白后重新定量。 ) [843] => Array ( [id] => 843 [name] => Allophycocyanin(APC)-Conjugated [url] => category-843-b0.html [cat_desc] => ) [844] => Array ( [id] => 844 [name] => CY Fluorescent-Conjugated [url] => category-844-b0.html [cat_desc] => ) [845] => Array ( [id] => 845 [name] => Phycoglobin(PE)-Conjugated [url] => category-845-b0.html [cat_desc] => ) [82] => Array ( [id] => 82 [name] => RBTIC-Conjugated [url] => category-82-b0.html [cat_desc] => 荧光TRITC标记类产品将RBITC(Sigma 产品)共价交联到抗体(IgG)分子的赖氨酸分子上,并经过纯化而来,每支0.5ml液体,含有0.03%NaN3防腐,IgG蛋白浓度在5mg/ml左右,RBITC约20-40μg/ml。最大吸收波长555nm,最大发射波长为600nm。工作浓度用0.01mol/L pH7.4PBS作1:50-1:500稀释使用。稀释抗体尽可能的在一周内用完。浓缩液-20℃可保存一年,尽量少反复冻融。 ) [79] => Array ( [id] => 79 [name] => Peroxidase (HRP)-Conjugated [url] => category-79-b0.html [cat_desc] => 过氧化物酶标记抗体,是一类广泛用于免疫学、分子生物学及临床医学的各个分支学科中的特异、敏感和安全的免疫化学制剂。本公司制备的各种HRP标记抗体,均根据改良的过碘酸钠氧化法标记而成。本产品每0.1ml液体含有HRP约200ug/支,IgG约含400ug/支。 工作效价: ELISA法:1:500~5000,用TMB作底物(OD450nm)P/N=2.5~3.0为阳性。 免疫印迹:1:200~5000。 免疫组化:1:20~500。 ) ) ) )
>
Antibodies >
Secondary Antibodies >
CY Fluorescent-Conjugated >
Rabbit Anti-Human IgG/Cy7English Name | Rabbit Anti-Human IgG/Cy7 |
Host | Rabbit |
Calculated MW | 160kDa |
Purification Method | affinity purified by Protein A |
Concentration | 2mg/mL |
Conjugation | Cy7 |
Piece | Piece |
Specification | 100ul |
Reactivity | Human IgG |
Application | IF |
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.