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My CartReactivity：Human
Application：WB,IHC,IP,ELISA
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：54541
Host：Rabbit
Qty：
Size：
| Name | Anti-DDIT4 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. IP 1:50-500. ELISA 1:2000-20000. |
| Target | DDIT4 |
| Background | Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death. |
| Swiss Prot | Q9NX09 |
| Gene ID | 54541 |
| Subcellular Location | Cytoplasm Mitochondrion Cytosol |
| Immunogen | Recombinant protein of human DDIT4 |
| Public Immunogen Range | 1-232aa |
| Calculated MW | 25kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. ELISA 1:2000-20000. |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human |
| Application | WB IHC ELISA IP |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with DDIT4 antibody diluted at 1:2000;Lane: A549,HeLa,K562,HMC1.
Immunohistochemistry of paraffin-embedded Human cervical cancer with DDIT4 antibody diluted at 1:200.
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