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My CartReactivity：Rat,Human,Mouse
Application：WB,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：229
Host：Rabbit
Qty：
Size：
| Name | Anti-ALDOB Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Sheep;Rabbit;Chimpanzee;Cow;Chicken |
| Dilution Ratio | WB 1:5000-8000. IHC 1:100-300. |
| Target | ALDOB |
| Background | Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. |
| Swiss Prot | P05062 |
| Gene ID | 229 |
| Subcellular Location | Cytoplasm centriolar satellite centrosome cytoskeleton microtubule organizing center |
| Immunogen | A synthetic peptide of human ALDOB |
| Public Immunogen Range | 10-60/364aa |
| Calculated MW | 39kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with ALDOB antibody diluted at 1:6000;Lane: Mouse liver,Mouse kidney,Rat liver,Rat kidney.
Immunohistochemistry of paraffin-embedded Human renal cancer with ALDOB antibody diluted at 1:200.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with ALDOB antibody diluted at 1:200.
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