Application: ELISA
Reactivity:Sheep
Sensitivity:4 pg/ml
Storage:2-8℃,6 months
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Application: ELISA
Reactivity:Sheep
Sensitivity:4 pg/ml
Storage:2-8℃,6 months
Chinese Name | 羊碱性成纤维细胞生长因子检测试剂盒 |
English Name | Sheep FGF basic ELISA Kit |
Synonyms | BFGF; FGFB; FGF-2; HBGF-2 |
Storage | 2-8℃,6 months |
Detection Target | FGF basic |
Sample Type | Serum,Plasma,Cell Culture Supernatant |
Reactivity | Sheep |
Application | ELISA |
Reaction Type | Sandwich-ELISA |
Quantitative/Qualitative | Quantitative |
Detection Range | 7.81-500 pg/ml |
Sensitivity | 4 pg/ml |
Specificity | Do not react with BMP1,BMP2,BMP3,BMP4,IL-1β,IL-4,IL-5,IL-6,IL-8,IFN-γ,TGF-β1,TGF-β2,TGF-β3,TLR1,TLR2,TLR3 etc. |
Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
Recovery | 80%-120% |
Assay Time | 2.33h |
Swiss Prot | P20003 |
Gene ID | 443306 |
Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗羊FGF basic单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的羊FGF basic会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗羊FGF basic抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的羊FGF basic发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的羊FGF basic,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的羊FGF basic浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中羊FGF basic的浓度。 |
Unit | Kit |
Specification | 48T 96T |