Application: ELISA
Reactivity:Chicken
Sensitivity:62 pg/ml
Storage:2-8℃,6 months
Array ( [61] => Array ( [id] => 61 [name] => ELISA [children] => Array ( [117] => Array ( [id] => 117 [name] => Human ) [158] => Array ( [id] => 158 [name] => Mouse ) [157] => Array ( [id] => 157 [name] => Rats ) [776] => Array ( [id] => 776 [name] => Common Species ) [159] => Array ( [id] => 159 [name] => Other Species ) [777] => Array ( [id] => 777 [name] => Unpackaged Kit ) [238] => Array ( [id] => 238 [name] => Antibody Pairs ) [754] => Array ( [id] => 754 [name] => Related Auxiliary Reagent ) ) [num] => 9 ) )
Application: ELISA
Reactivity:Chicken
Sensitivity:62 pg/ml
Storage:2-8℃,6 months
Chinese Name | 鸡胰岛素样生长因子1检测试剂盒 |
English Name | Chicken IGF-1 ELISA Kit |
Synonyms | IBP1; IGF1A; IGFI; IGF-IA; IGF-IB; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C |
Storage | 2-8℃,6 months |
Detection Target | IGF-1 |
Sample Type | Serum,Plasma,Cell Culture Supernatant |
Reactivity | Chicken |
Application | ELISA |
Reaction Type | Sandwich-ELISA |
Quantitative/Qualitative | Quantitative |
Detection Range | 125-8000 pg/ml |
Sensitivity | 62 pg/ml |
Specificity | Do not react with Chicken IGF-1 analogue etc. |
Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
Recovery | 80%-120% |
Assay Time | 2.33h |
Swiss Prot | P18254 |
Gene ID | 418090 |
Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗鸡IGF-1单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的鸡IGF-1会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗鸡IGF-1抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的鸡IGF-1发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的鸡IGF-1,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的鸡IGF-1浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中鸡IGF-1的浓度。 |
Background | Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine,paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human,bovine and porcine proteins (100% identity),and exhibit cross-species activity. |
Unit | Kit |
Specification | 48T 96T |