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My CartReactivity：Human,Mouse,Rat
Application：WB,IF,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：7124
Host：Rabbit
Qty：
Size：
| Name | Anti-TNF Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. IHC 1:500-1000. IF 1:50-100. |
| Target | TNF |
| Background | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| Swiss Prot | P01375 |
| Gene ID | 7124 |
| Subcellular Location | Cell membrane Membrane Secreted Single-pass type II membrane protein Single-pass type II membrane protein |
| Immunogen | Recombinant protein of human TNF |
| Public Immunogen Range | 134-233aa |
| Calculated MW | 26kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.03% Proclin300，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with TNF antibody diluted at 1:1000;Lane: 293T,HT-1080
Immunohistochemistry of paraffin-embedded Human lung cancer with TNF antibody diluted at 1:100
Immunofluorescence analysis of L929 cells using TNF antibody diluted at 1:100.
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