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CAS	56-41-7
Chinese Name	L-丙氨酸
English Name	L-Alanine
Synonyms	alanine;L-2-Aminopropionic acid
Molecular Formula	C₃H₇NO₂
Molecular Weight	89.09
Solubility	Soluble in Water ≥50mg/mL
Storage	Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity	HPLC≥98%
Appearance	White to off-white Solid
EC	EINECS 200-273-8
MDL	MFCD00064410
SMILES	N[C@@H](C)C(O)=O
InChIKey	QNAYBMKLOCPYGJ-REOHCLBHSA-N
InChI	InChI=1S/C3H7NO2/c1-2(4)3(5)6/h2H,4H2,1H3,(H,5,6)/t2-/m0/s1
PubChem CID	5950
Target Point	Others
Passage	Others
Background	It is a non-essential amino acid involved in the metabolism of sugars and acids and provides energy for muscle tissue, brain and central nervous system.
Biological Activity	L-Alanine 是一种非必需氨基酸，能够参与糖和酸的代谢，增强免疫力，并能为肌肉组织、大脑和中枢神经系统提供能量[1]。
In Vitro	hiPSC，201B7细胞和ehiPSC的活力随着L-Alanine 浓度的增加而降低，并且在1.2M L-Alanine 时分别达到7.5±1.3％和3.7±0.7％。另一方面，未观察到hFB和hSkMC的存活率降低。虽然随着L-Alanine 浓度的增加，iCM的活力略有下降，但iCM在1.2 M浓度的L-Alanine 的存活率为49.4±6.9％，显著高于未分化的iPSC，201B7细胞和ehiPSC(p <0.01)。即使在处理2或4小时后，hiPSC，201B7细胞和ehiPSC的存活率也急剧下降。相反，hFB的存活率在1，2和4小时未能降低，并且在24小时处理时显示出小的降低。在用1.2ML-Alanine 处理2小时后，悬浮培养中201B7细胞的存活率降低至11.8±6.0％，而hFB的存活率为72.9±14.2％[1]。
Cell Experiment	为了研究通过高L-Alanine 培养基从分化细胞中选择性去除hiPSC，使用两种类型的hiPSC，201B7 hiPSC(201B7细胞)和通过附加型系统(ehiPSC)衍生的hiPSC系，以及正常人皮肤成纤维细胞(hFBs)，人骨骼肌细胞(hSkMCs)和hiPSC衍生的心肌细胞(iCMs)作为分化细胞。将细胞在补充有各种浓度(0-1.2M)或处理时间(1-24小时)的L-Alanine 的培养基中温育。用普通培养基替换培养基，24小时后测量相对细胞活力[1]。
Data Literature Source	[1]. Nagashima T，et al. Selective Elimination of Human Induced Pluripotent Stem Cells Using Medium with High Concentration of L-Alanine. Sci Rep. 2018 Aug 20;8(1):12427.
Unit	Piece
Specification	200mg 10mM*1mL in Water 500mg 1g