Application: ELISA
Reactivity:Porcine
Sensitivity:3 pg/ml
Storage:2-8℃,6 months
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Application: ELISA
Reactivity:Porcine
Sensitivity:3 pg/ml
Storage:2-8℃,6 months
Chinese Name | 猪单核细胞趋化蛋白1检测试剂盒 |
English Name | Porcine CCL2/MCP-1 ELISA Kit |
Synonyms | Monocyte Chemotactic Protein 1; C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF |
Storage | 2-8℃,6 months |
Detection Target | CCL2/MCP-1 |
Sample Type | Serum,Plasma,Cell Culture Supernatant |
Reactivity | Porcine |
Application | ELISA |
Reaction Type | Sandwich-ELISA |
Quantitative/Qualitative | Quantitative |
Detection Range | 6.25-400 pg/ml |
Sensitivity | 3 pg/ml |
Specificity | Do not react with ApoAI,BMP1,BMP2,BMP3,BMP4,BMP5,BMP7,CRP,FGFacidic,HGF,HSP27,IL-1α,IL-1β,IL-1RA,IL-1RI,IL-2,IL-5,IL-6,IL-8,IL-10,IL-12,IL-13,IL-15,IL-17C,IL-21,IGF1,MMP-2,MMP9,PDGF,PLA2G7,sIL-2R,sIL-6R,TGFβ1,TGFβ2,TGFβ3,TLR1,TLR2,TLR3,TNF-α,TNFRI,TNFRII,VEGF etc. |
Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
Recovery | 80%-120% |
Assay Time | 2.33h |
Swiss Prot | P42831 |
Gene ID | 397422 |
Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗猪CCL2单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的猪CCL2会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗猪CCL2抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的猪CCL2发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的猪CCL2,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的猪CCL2浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中猪CCL2的浓度。 |
Background | CCL2/JE/MCP-1,is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes,NK cells,lymphocytes,and basophils. Additionally,CCL2 promotes Th2 polarization in CD4+ T cells,and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis,multiple sclerosis,and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus,including the N-terminal pyrrolidone carboxylic acid-modified glutamine,downregulates activity but not receptor binding. |
Unit | Kit |
Specification | 48T 96T |