Application: ELISA
Reactivity:Porcine
Sensitivity:1.5pg/mL
Storage:2-8℃,6 months
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Application: ELISA
Reactivity:Porcine
Sensitivity:1.5pg/mL
Storage:2-8℃,6 months
Chinese Name | 猪催乳素检测试剂盒 |
English Name | Porcine Prolactin ELISA Kit |
Synonyms | PRL; Prolactin; Lactotrope; LTH; Luteotropic Hormone |
Storage | 2-8℃,6 months |
Detection Target | Prolactin |
Sample Type | Serum,Plasma,Cell Culture Supernatant |
Reactivity | Porcine |
Application | ELISA |
Reaction Type | Sandwich-ELISA |
Quantitative/Qualitative | Quantitative |
Detection Range | 40-2600pg/mL |
Sensitivity | 1.5pg/mL |
Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
Recovery | 80%-120% |
Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗猪prolactin单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的猪prolactin会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗猪prolactin抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的猪prolactin发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的猪prolactin,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的猪prolactin浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中猪prolactin的浓度。 |
Background | Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary,placenta,brain,uterus,dermal fibroblasts,decidua,B cells,T cells,natural killer cells,and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family. |
Unit | Kit |
Specification | 48T 96T |